Queensland Bladder Cancer Initiative (QBCI)

1 ARTICLES PUBLISHED IN JoVE

Cancer Research

Culture of Bladder Cancer Organoids as Precision Medicine Tools

Patrick B. Thomas^1,2,3,4, Mahasha P. J. Perera^1,2,3,4,5, Saeid Alinezhad^1,4, Andre Joshi^1,4,5, Paria Saadat^1,2, Clarissa Nicholls^1,2, Caitlin P. Devonport^1,2,4, Alivia R. Calabrese^1,2,3,4, Abby R. Templeton^1,2,3, Jack R. Wood^1,2, Nathan J. Mackenzie^1,2, Penny L. Jeffery^1,2,3,4, Ian Vela^*1,2,3,4,5, Elizabeth D. Williams^*1,2,3,4

¹School of Biomedical Sciences, Faculty of Health, Queensland University of Technology (QUT) at Translational Research Institute, ²Queensland Bladder Cancer Initiative (QBCI), ³Centre for Personalised Analysis of Cancers (CPAC), ⁴Australian Prostate Cancer Research Centre - Queensland, ⁵Department of Urology, Princess Alexandra Hospital

Patient-derived organoids (PDOs) are a powerful tool in translational cancer research, reflecting both the genetic and phenotypic heterogeneity of the disease and response to personalized anti-cancer therapies. Here, a consolidated protocol to generate human primary bladder cancer PDOs in preparation for the evaluation of phenotypic analyses and drug responses is detailed.